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In vivo EPRを用いた紫外線照射したマウスの皮膚におけるフリーラジカル生成の評価
https://doi.org/10.32150/00010837
https://doi.org/10.32150/00010837a7b67fd8-db0c-4220-9416-178bfbdc66e7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
73-1.2-a18.pdf (397.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-02-09 | |||||
| タイトル | ||||||
| タイトル | In vivo EPRを用いた紫外線照射したマウスの皮膚におけるフリーラジカル生成の評価 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 活性酸素種 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 酸化還元バランス | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ナイトロオキサイド | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 半減期 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 塗布 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.32150/00010837 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
神林, 勲
× 神林, 勲× 塚本, 未来× 木本, 理可× 東郷, 将成× 秋月, 茜× 沢村, 祥子× 内田, 英二 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27664 | |||||
| 姓名 | KAMBAYASHI, Isao | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27665 | |||||
| 姓名 | TSUKAMOTO, Miku | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27666 | |||||
| 姓名 | KIMOTO, Rika | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27667 | |||||
| 姓名 | TOGO, Masanari | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27668 | |||||
| 姓名 | AKIZUKI, Akane | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27669 | |||||
| 姓名 | SAWAMURA, Shoko | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 27670 | |||||
| 姓名 | UCHIDA, Eiji | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study was designed to investigate the generation of free radicals (including reactive oxygen species) during exposure to ultraviolet light (UV) in intact mouse skin by an in vivo EPR (electron paramagnetic resonance) spectrometer. Two kinds of nitroxyl probes, TEMPONE (4-oxo-2,2,6,6-tetramethyl-piperidine-d16-1-oxyl) and CMP (3-carbamoyl- 2,2,5,5-tetramethylpyrrolidine-N-oxyl), were injected intraperitoneal and/or directly supplicated to skin. Signal decays of nitroxyl probes were monitored with the in vivo EPR spectrometer equipped with a surface-coil-resonator after UV (UVA＋B) irradiation for 3 minutes. In the direct skin supplication study, there was no significant difference between the control and UV-irradiation groups in half-lives (sec.) of TEMPONE. However, half-lives of CMP were significantly prolonged in the UV-irradiation group compared with the control group, and the difference was substantially eliminated with a spin-trapping agent (PBN, N-tert-butyl-α-phenylnitrone) before UV-irradiation. In the intraperitoneal injection study, half-lives of CMP measured in the skin were significantly prolonged in the UVirradiation group compared to that of the control group. Our results suggest that direct supplicating the nitroxyl probe to the skin is useful as an extremely noninvasive method to evaluate free radical generation after UV-irradiation. | |||||
| 書誌情報 |
北海道教育大学紀要. 基礎研究編 巻 73, 号 1・2, p. 217-225, 発行日 2023-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 27583899 | |||||
| 書誌レコードID | ||||||
| 識別子タイプ | NCID | |||||
| 関連識別子 | AB00000251 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他のタイトル | ||||||
| その他のタイトル | In vivo EPR Studies on Free Radicals Generation in UV-irradiated Mouse Skin | |||||
| 出版者 | ||||||
| 出版者 | 北海道教育大学 | |||||
