This study examines the relationship between total oxygen-consumption （ΣVO2） during exercise and exercise-induced oxidative stress （EIOS） during moderate aerobic exercise. Urinary 8-hydroxy-2’-deoxyguanosine （8-OHdG） level and serum redox balance with electron paramagnetic resonance/nitroxide spin probe method were used as biomarkers of EIOS. Twelve healthy active men［age: 20.3 ± 1.9 yr, height: 178.7 ± 5.1 cm, weight: 73.8 ± 7.6 kg, BMI: 23.4 ± 1.9 kg/（m）2］participated in this study after providing written informed consent. The subjects performed incremental exercises to volitional exhaustion to measure peak oxygen uptake （VO2peak） using a bicycle ergometer. They also exercised on separate days for 30 min （30EX）, 60 min （60EX） and 90 min （90EX） at an identical intensity of 50 ％ VO2peak on the bicycle ergometer. Cardio-respiratory data were measured during theexercise. Urine samples were collected before （baseline） and after （1.5 h） the exercise, and blood samples were collected at baseline, immediately after exercise （0 h）, and 1.5 h after exercise. The ΣVO2 were 746.3 ± 74.7 ml/kg, 1580.9 ± 78.0 ml/kg and 2554.5 ± 169.9 ml/ kg in 30EX, 60EX and 90EX, respectively. However, urinary 8-OHdG levels and serum redox balance did not change before and after each exercise. These results suggest that ΣVO2 during exercise does not have a significant effect on EIOS in moderate aerobic exercise for up to 90min.
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